Stabilization of a recombinant ricin toxin A subunit vaccine through lyophilization
نویسندگان
چکیده
منابع مشابه
Immunomodulatory Activity of Recombinant Ricin Toxin Binding Subunit B (RTB)
Ricin toxin binding subunit B (RTB) is one of the subunits of the ricin protein. RTB has been used as adjuvant, but little is known about its mechanism. In this study, we found that RTB increased not only nitric oxide (NO) release, but also tumor necrosis factor (TNF)-α and interleukin (IL)-6 production in mouse macrophage cell line RAW264.7 cells. They subsequently exhibited enhanced ConA-indu...
متن کاملRiVax, a recombinant ricin subunit vaccine, protects mice against ricin delivered by gavage or aerosol.
Ricin is a plant toxin that is a CDC level B biothreat. Our recombinant ricin A chain vaccine (RiVax), which contains mutations in both known toxic sites, has no residual toxicity at doses at least 800 times the immunogenic dose. RiVax without adjuvant given intramuscularly (i.m.) protected mice against intraperitoneally administered ricin. Furthermore the vaccine without alum was safe and immu...
متن کاملStabilization of botulinum toxin type A during lyophilization.
Botulinum toxin for medical use is diluted to very low concentrations (nanograms per milliliter); when it is preserved by lyophilization, considerable loss of activity can occur. In the present study, conditions that gave > 90% recovery of the toxicity after lyophilization of solutions containing 20 to 1,000 mouse 50% lethal doses per ml were found. Toxicity was recovered upon drying 0.1 ml of ...
متن کاملStructure of RiVax: a recombinant ricin vaccine
RiVax is a recombinant protein that is currently under clinical development as part of a human vaccine to protect against ricin poisoning. RiVax includes ricin A-chain (RTA) residues 1-267 with two intentional amino-acid substitutions, V76M and Y80A, aimed at reducing toxicity. Here, the crystal structure of RiVax was solved to 2.1 Å resolution and it was shown that it is superposable with that...
متن کاملrecombinant toxin-coregulated pilus a (tcpa) as a candidate subunit cholera vaccine.
background and objectives: the toxin co-regulated pilus a (tcpa) has been described as a criti- cal pathogenicity factor of vibrio cholerae . tcpa is a candidate for making subunit vaccine against chol- era. the aim of this study was to produce a candidate vaccine by expressing recombinant tcpa in e. coli . materials and methods: i...
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ژورنال
عنوان ژورنال: European Journal of Pharmaceutics and Biopharmaceutics
سال: 2013
ISSN: 0939-6411
DOI: 10.1016/j.ejpb.2013.03.029